Microsatellite Markers to Determine Population Genetic Structure in the Golden Anchovy, Coilia dussumieri

Kathirvelpandian, A and Gopalakrishnan, A and Lakra, W S and Krishna, Gopal and Sharma, R and Musammilu, K K and Basheer, V S and Jena, J K (2014) Microsatellite Markers to Determine Population Genetic Structure in the Golden Anchovy, Coilia dussumieri. Biochemical Genetics, 52 (5-6). pp. 296-309.

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    Abstract

    Coilia dussumieri (Valenciennes, 1848) commonly called as golden anchovy, constitutes a considerable fishery in the northern part of both the west and east coasts of India. Despite its clear-cut geographic isolation, the species is treated as a unit stock for fishery management purposes. We evaluated 32 microsatellite primer pairs from three closely related species (resource species) belonging to the family Engraulidae through cross-species amplification in C. dussumieri. Successful cross-priming was obtained with 10 loci, which were sequenced for confirmation of repeats. Loci were tested for delineating the genetic stock structure of four populations of C. dussumieri from both the coasts of India. The number of alleles per locus ranged from 8 to 18, with a mean of 12.3. Results of pairwise FST indicated genetic stock structuring between the east and west coast populations of India and also validated the utilization of identified microsatellite markers in population genetic structure analysis.

    Item Type: Article
    Uncontrolled Keywords: Coilia dussumieri, Cross-species amplification, Microsatellite validation, Population genetic structure
    Subjects: Fish Genetics
    Divisions: CMFRI-Kochi > Marine Biotechnology
    Subject Area > CMFRI > CMFRI-Kochi > Marine Biotechnology
    CMFRI-Kochi > Marine Biotechnology
    Subject Area > CMFRI-Kochi > Marine Biotechnology
    Depositing User: Arun Surendran
    Date Deposited: 27 Jun 2015 10:05
    Last Modified: 09 Sep 2015 16:01
    URI: http://eprints.cmfri.org.in/id/eprint/10440

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