Molecular phylogenetics of three species of the genus Rastrelliger using mitochondrial DNA markers

Basheer, V S and Mohitha, C and Vineesh, N and Gopalakrishnan, A and Jena, J K (2015) Molecular phylogenetics of three species of the genus Rastrelliger using mitochondrial DNA markers. Molecular Biology Reports, 42 (4). pp. 873-879.

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Abstract

In the present study three species of mackerel, Rastrelliger present in Indian waters were taken for geneticidentification using cytochrome c oxidase subunit I and 16srRNA sequences. Accurate identification of these species isimportant for fishery management as its morphologicalcharacters are very similar. In this study, the sequences of COI and 16S rRNA were determined from 19 individualsof three Rastrelliger species, Rastrelliger kanagurta, Ra-strelliger brachysoma and Rastrelliger faughni fromAndamans and Indian mainland to study the phylogeneticrelationship. The intraspecies and interspecies geneticdistance ranged from 0.000 to 0.002 and 0.007 to 0.015respectively based on 16S rRNA sequences. Using COIdata analysis, the intraspecies genetic distance ranged from0.000 to 0.012, while it varied from 0.039 to 0.086 forinterspecies. The present study clearly demarcates threespecies of mackerel based on the mitochondrial geneticsequences and also showed a non-descriptive genetic dis-tance of R. kanagurta from mainland and AndamanIslands.

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