Development of marine fish cell lines and stem cell lines: applications in mariculture and marine biodiversity

Sobhana, K S (2015) Development of marine fish cell lines and stem cell lines: applications in mariculture and marine biodiversity. [Teaching Resource]

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    Abstract

    In vitro transformed/continuous marine fish cell lines are important for virology, gene expression studies, cytogenetics, as in vitro models in toxicology, transgenics, in many other basic studies and in biodiversity conservation. In vitro cell culture systems/cell lines derived from marine fish are necessary for isolation and characterisation of viruses and studies on diversity of viruses in marine environment. The main impetus for the development of many of the continuous fish cell lines was to provide the means for isolating and identifying viruses that are the causative agents of epizootics of commercially important species. Unlike other microorganisms, which can be readily grown in artificial nutrient medium, viruses are obligatory intracellular pathogens and their isolation and propagation are totally dependent on the availability of a live host, such as permissive cell cultures. In addition, most viruses are host-specific and tissue-specific, and they can only be isolated and propagated using cell lines established from tissues of the same/related host species. An appropriate cell line is the most important laboratory tool to grow, isolate, characterise, and identify pathogenic fish viruses. With few exceptions, fish viruses can be replicated only in fish cell cultures.

    Item Type: Teaching Resource
    Uncontrolled Keywords: marine fish cell lines; stem cell lines; mariculture; marine biodiversity
    Subjects: Aquaculture > Mariculture
    Fish Biotechnology > Microbiology
    Marine Biodiversity
    Divisions: CMFRI-Kochi > Biodiversity
    Subject Area > CMFRI > CMFRI-Kochi > Biodiversity
    CMFRI-Kochi > Biodiversity
    Subject Area > CMFRI-Kochi > Biodiversity
    Depositing User: Arun Surendran
    Date Deposited: 23 Jun 2015 06:09
    Last Modified: 09 Sep 2015 16:01
    URI: http://eprints.cmfri.org.in/id/eprint/10432

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