Evaluation of α- and β-glucosidase inhibitory properties of macro-algae using intestinal extracts of marine snail, Thais rudolphi (Lamarck, 1822)

Pramitha, V S and Lipton, A P and Thangaraj, M (2008) Evaluation of α- and β-glucosidase inhibitory properties of macro-algae using intestinal extracts of marine snail, Thais rudolphi (Lamarck, 1822). Indian Journal of Biotechnology, 7 (1). pp. 66-72.

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    Abstract

    Glucosidase-inhibitory activity of marine macro-algae, Ulva fasciata and Hypnea musciformis in different solvents was detected using enzyme-agar plate method. Eenzyme-agar plate containing α- or β-D-glucosidase was used in combination with substrate-agar plate containing p-nitro phenyl α- and β-D-glucopyranoside (PNPG). Discs impregnated with algal extracts placed on the enzyme agar, followed by pre-incubation up to 2.5 h at 25°C, resulted in the characteristic inhibitory circle around the inhibitor. Extracts of red algae, H. musciformis had higher glucosidase-inhibition than the extracts of U. fasciata. The intestine of Thais rudolphi contained both α- and β-glucosidase enzymes and their inhibition was comparable with the commercial enzymes, suggesting the possible alternate method for assessing the glucosidase-inhibitory activity of the marine macro-algae

    Item Type: Article
    Uncontrolled Keywords: Glucosidases; Hypnea musciformis; macro-algal inhibitors; rapid assay; Thais rudolphi; Ulva fasciata
    Subjects: Molluscan Fisheries
    Fish Biotechnology > Bioactive compound
    Divisions: CMFRI-Vizhinjam
    Depositing User: Arun Surendran
    Date Deposited: 29 Jul 2014 04:55
    Last Modified: 09 Sep 2015 15:59
    URI: http://eprints.cmfri.org.in/id/eprint/10140

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